Effect of delayed serum separation on various chemistry analytes.

Objective: To observe the effect of delayed serum separation on various chemistry analytes. Study Design: Quasi Experimental study. Setting: Clinical Chemistry Laboratory, Sheikh Zayed Medical College & Hospital, Rahim Yar Khan. Period: 1st December 2021 to 31st January 2022. Material Methods: Fifty healthy volunteers age 25-55 years from both genders were included in study using consecutive sampling technique. Informed verbal consent was taken all subjects. 4 cc blood drawn each subject and divided into 3 plain tubes. 1 tube analyzed at hour for glucose, urea, creatinine, ALT, AST, ALP, sodium, potassium, chloride calcium. 2nd same analytes 2 hours after sample collection 3rd hours. Results recorded a predesigned performa. Data entered SPSS Software 23 Windows. presented terms mean SD. P value p < 0.05 considered significant. Results: Glucose, potassium showed statistically significant variation over time while others remained stable up There decrease glucose (p=0.000) increase observed sodium (p=0.0001), (p=0.0001) ALT (p=0.002). Conclusion: Blood samples should be transported laboratory immediately when received centrifuged processed within as transportation affects many may lead erroneous test results.